Spo0A, the key transcriptional regulator for entrance into sporulation, is an inhibitor of DNA replication
Identifieur interne : 000963 ( Istex/Checkpoint ); précédent : 000962; suivant : 000964Spo0A, the key transcriptional regulator for entrance into sporulation, is an inhibitor of DNA replication
Auteurs : Virginia Castilla-Llorente ; Daniel Mu Oz-Espín ; Laurentino Villar ; Margarita Salas ; Wilfried J J. Meijer [Espagne]Source :
- The EMBO Journal [ 0261-4189 ] ; 2006-08-23.
English descriptors
- Teeft :
- Acad, Bacillus, Bacillus subtilis, Biol, Biology organization spo0a, Blanco, Chromosomal, Chromosome, Coli, Dilution steps, Dimer, Dnaa, Dnaa boxes, Dnase, Embo, Embo journal, Footprint, Footprinting, Fujita, Genome, Imperfect dnaa boxes, Inca, Incb, Incb region, Incc, Inhibitor, Inhibitory effect, Initiation, Initiation step, Krause, Ladds, Losick, Lytic cycle, Meijer, Microbiol, Mismatch, Moriya, Muchova, Natl, Oric, Phage, Phosphorylated, Plasmid, Polymerase, Proc, Proc natl acad, Promoter, Regulator, Replication, Replication initiation, Replication initiator protein, Salas, Spo0a, Spo0a concentrations, Sporulation, Subtilis, Subtilis chromosome, Subtilis oric, Subtilis oric region, Transcriptional, Virulent phage.
Abstract
The transcription factor Spo0A is a master regulator for entry into sporulation in Bacillus subtilis and also regulates expression of the virulent B. subtilis phage ϕ29. Here, we describe a novel function for Spo0A, being an inhibitor of DNA replication of both, the ϕ29 genome and the B. subtilis chromosome. Binding of Spo0A near the ϕ29 DNA ends, constituting the two origins of replication of the linear ϕ29 genome, prevents formation of ϕ29 protein p6‐nucleoprotein initiation complex resulting in inhibition of ϕ29 DNA replication. At the B. subtilis oriC, binding of Spo0A to specific sequences, which mostly coincide with DnaA‐binding sites, prevents open complex formation. Thus, by binding to the origins of replication, Spo0A prevents the initiation step of DNA replication of either genome. The implications of this novel role of Spo0A for phage ϕ29 development and the bacterial chromosome replication during the onset of sporulation are discussed.
Url:
DOI: 10.1038/sj.emboj.7601266
Affiliations:
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Meijer</name><affiliation wicri:level="1"><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Biología Molecular ‘Eladio Viñuela’ (CSIC), Centro de Biología Molecular ‘Severo Ochoa’ (CSIC‐UAM), Universidad Autónoma, Madrid, Canto Blanco</wicri:regionArea><wicri:noRegion>Canto Blanco</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Espagne</country></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">The EMBO Journal</title><title level="j" type="alt">THE EMBO JOURNAL</title><idno type="ISSN">0261-4189</idno><idno type="eISSN">1460-2075</idno><imprint><biblScope unit="vol">25</biblScope><biblScope unit="issue">16</biblScope><biblScope unit="page" from="3890">3890</biblScope><biblScope unit="page" to="3899">3899</biblScope><biblScope unit="page-count">10</biblScope><publisher>John Wiley & Sons, Ltd</publisher><pubPlace>Chichester, UK</pubPlace><date type="published" when="2006-08-23">2006-08-23</date></imprint><idno type="ISSN">0261-4189</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0261-4189</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acad</term><term>Bacillus</term><term>Bacillus subtilis</term><term>Biol</term><term>Biology organization spo0a</term><term>Blanco</term><term>Chromosomal</term><term>Chromosome</term><term>Coli</term><term>Dilution steps</term><term>Dimer</term><term>Dnaa</term><term>Dnaa boxes</term><term>Dnase</term><term>Embo</term><term>Embo journal</term><term>Footprint</term><term>Footprinting</term><term>Fujita</term><term>Genome</term><term>Imperfect dnaa boxes</term><term>Inca</term><term>Incb</term><term>Incb region</term><term>Incc</term><term>Inhibitor</term><term>Inhibitory effect</term><term>Initiation</term><term>Initiation step</term><term>Krause</term><term>Ladds</term><term>Losick</term><term>Lytic cycle</term><term>Meijer</term><term>Microbiol</term><term>Mismatch</term><term>Moriya</term><term>Muchova</term><term>Natl</term><term>Oric</term><term>Phage</term><term>Phosphorylated</term><term>Plasmid</term><term>Polymerase</term><term>Proc</term><term>Proc natl acad</term><term>Promoter</term><term>Regulator</term><term>Replication</term><term>Replication initiation</term><term>Replication initiator protein</term><term>Salas</term><term>Spo0a</term><term>Spo0a concentrations</term><term>Sporulation</term><term>Subtilis</term><term>Subtilis chromosome</term><term>Subtilis oric</term><term>Subtilis oric region</term><term>Transcriptional</term><term>Virulent phage</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract">The transcription factor Spo0A is a master regulator for entry into sporulation in Bacillus subtilis and also regulates expression of the virulent B. subtilis phage ϕ29. Here, we describe a novel function for Spo0A, being an inhibitor of DNA replication of both, the ϕ29 genome and the B. subtilis chromosome. Binding of Spo0A near the ϕ29 DNA ends, constituting the two origins of replication of the linear ϕ29 genome, prevents formation of ϕ29 protein p6‐nucleoprotein initiation complex resulting in inhibition of ϕ29 DNA replication. At the B. subtilis oriC, binding of Spo0A to specific sequences, which mostly coincide with DnaA‐binding sites, prevents open complex formation. Thus, by binding to the origins of replication, Spo0A prevents the initiation step of DNA replication of either genome. The implications of this novel role of Spo0A for phage ϕ29 development and the bacterial chromosome replication during the onset of sporulation are discussed.</div></front></TEI><affiliations><list><country><li>Espagne</li></country></list><tree><noCountry><name sortKey="Castilla Lorente, Virginia" sort="Castilla Lorente, Virginia" uniqKey="Castilla Lorente V" first="Virginia" last="Castilla-Llorente">Virginia Castilla-Llorente</name><name sortKey="Mu Oz Spin, Daniel" sort="Mu Oz Spin, Daniel" uniqKey="Mu Oz Spin D" first="Daniel" last="Mu Oz-Espín">Daniel Mu Oz-Espín</name><name sortKey="Salas, Margarita" sort="Salas, Margarita" uniqKey="Salas M" first="Margarita" last="Salas">Margarita Salas</name><name sortKey="Villar, Laurentino" sort="Villar, Laurentino" uniqKey="Villar L" first="Laurentino" last="Villar">Laurentino Villar</name></noCountry><country name="Espagne"><noRegion><name sortKey="Meijer, Wilfried J J" sort="Meijer, Wilfried J J" uniqKey="Meijer W" first="Wilfried J J" last="Meijer">Wilfried J J. Meijer</name></noRegion><name sortKey="Meijer, Wilfried J J" sort="Meijer, Wilfried J J" uniqKey="Meijer W" first="Wilfried J J" last="Meijer">Wilfried J J. Meijer</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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